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Image Search Results
Journal: PLoS ONE
Article Title: Molecular studies into cell biological role of Copine-4 in Retinal Ganglion Cells
doi: 10.1371/journal.pone.0255860
Figure Lengend Snippet: (A, B) Intraocular injections of AAV1-Cpne4 viruses were done in P0 or P14 Brn3b Cre/WT mice eyes. Blebs (indicated by boxes and arrows ) were seen occasionally on dendrites of Cpne4 infected RGCs. For controls, AAV1-eGFP was similarly injected in P0 Brn3b Cre/WT mice (C, D). No blebs were observed in the control infected RGCs. Airy scan images for one of the blebs (A’) and adjoining dendrites (A”). (E) Measurement of the ‘bleb’ areas for Cpne4 infected RGCs as compared to regular varicosities on controls shows a significant difference between them while no difference between P0 and P15 Cpne4 infected retinas. (F, G) Areas and lamination measurements indicate no differences between the Cpne4 transfected RGCs as compared to control transfected RGCs. (ID and OD: Inner and outer distance- distance between innermost or outermost tip of RGC dendrites and the INL; IPL: Width of the IPL). Scale bar for A-C: 50μm; Scale bar for A’, A”: 5μm.***, p< 0.001.
Article Snippet: Intraocular injections of 0.5 ìl (1e9 viral particles/ìl) of AAV1-Cpne4 or
Techniques: Infection, Injection, Transfection